Journal: Current Research in Food Science

Article Title: Following the fate of polystyrene micro and nanobeads during in vitro digestion

doi: 10.1016/j.crfs.2025.101086

Figure Lengend Snippet: Cell viability (%) of confluent Caco-2 cells after 24 h incubation with 1 μm or 60 nm polystyrene latex particles, 25–500 μg/mL diluted in DMEM complete medium. Control cells were grown in DMEM complete medium with no treatment (100 % viability). Values are the average of six replicates; bars represent standard deviations.

Article Snippet: Polystyrene latex particles (PS) of 60 nm and 1 μm (10 % w/v) were supplied by MAGSPHERE INC (Pasadena, California, USA).

Techniques: Incubation, Control

Journal: Current Research in Food Science

Article Title: Following the fate of polystyrene micro and nanobeads during in vitro digestion

doi: 10.1016/j.crfs.2025.101086

Figure Lengend Snippet: Caco-2 cell count per well (A) in 24 well/plates after cumulative exposure to 500 μg/mL of 1 μm or 60 nm polystyrene latex particles during 24, 48 or 72 h. Control cells were grown in DMEM complete medium with no treatment. Culture media and treatments were refreshed every 24 h. Values are the average of three replicates; bars represent standard deviations (B) Optical microscope images of Caco-2 cells after 72 h treatment (4x objective).

Article Snippet: Polystyrene latex particles (PS) of 60 nm and 1 μm (10 % w/v) were supplied by MAGSPHERE INC (Pasadena, California, USA).

Techniques: Cell Counting, Control, Microscopy

Journal: Current Research in Food Science

Article Title: Following the fate of polystyrene micro and nanobeads during in vitro digestion

doi: 10.1016/j.crfs.2025.101086

Figure Lengend Snippet: Reducing SDS-PAGE protein profile (A) before and after simulated gastrointestinal digestion (SGID) of whey protein isolate (WPI) and SGID WPI formulations containing 1 μm or 60 nm polystyrene latex particles. A control sample with H2O was also subjected to the same SGID process. CLMS micrographs (B) of digested WPI formulations containing 1 μm or 60 nm polystyrene latex particles. The green colour represents protein and the scale bars are 5 μm.

Article Snippet: Polystyrene latex particles (PS) of 60 nm and 1 μm (10 % w/v) were supplied by MAGSPHERE INC (Pasadena, California, USA).

Techniques: SDS Page, Control